DNA

Part:BBa_M36560:Design

Designed by: Michael Raitor   Group: Stanford BIOE44 - S11   (2014-10-24)


cDNA of ESR2 Gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 30
    Illegal PstI site found at 1174
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 30
    Illegal PstI site found at 1174
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 30
    Illegal BglII site found at 1124
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 30
    Illegal PstI site found at 1174
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 30
    Illegal PstI site found at 1174
    Illegal NgoMIV site found at 150
    Illegal AgeI site found at 1522
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In finding the ESR2 coding sequence, we had to be sure to use the cDNA sequence as that would not need to be cut apart as a full cDNA sequence would. Using the cDNA sequence made our part shorter and increased its versatility, as it could now be used in prokaryotic cells.


Source

This sequence comes from human DNA, but the sequence used in the part is the cDNA format in oder to avoid the necessity of splicing the mRNA produced before translation (something that prokaryotic cells are not capable of). The cDNA sequence was obtained from the website www.sinobiological.com and the link to the specific page is listed here: http://www.sinobiological.com/ESR2-cDNA-Clone-g-2579.html

References